Pubmed-entry ::= {
  pmid 22187035,
  medent {
    em std {
      year 2012,
      month 2,
      day 1
    },
    cit {
      title {
        name "A pilot study to explore circulating tumour cells in pancreatic
 cancer as a novel biomarker."
      },
      authors {
        names ml {
          "Khoja L",
          "Backen A",
          "Sloane R",
          "Menasce L",
          "Ryder D",
          "Krebs M",
          "Board R",
          "Clack G",
          "Hughes A",
          "Blackhall F",
          "Valle JW",
          "Dive C"
        },
        affil str "Clinical and Experimental Pharmacology Group, Paterson
 Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK."
      },
      from journal {
        title {
          iso-jta "Br. J. Cancer",
          ml-jta "Br J Cancer",
          issn "1532-1827",
          name "British journal of cancer"
        },
        imp {
          date std {
            year 2012,
            month 1,
            day 31
          },
          volume "106",
          issue "3",
          pages "508-516",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus aheadofprint,
              date std {
                year 2011,
                month 12,
                day 20
              }
            },
            {
              pubstatus other,
              date std {
                year 2011,
                month 12,
                day 22,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2011,
                month 12,
                day 22,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2012,
                month 3,
                day 28,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus other,
              date std {
                year 2013,
                month 1,
                day 31,
                hour 0,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pii "bjc2011545",
        doi "10.1038/bjc.2011.545",
        pubmed 22187035,
        other {
          db "pmc",
          tag str "PMC3273340"
        },
        other {
          db "ELocationID doi",
          tag str "10.1038/bjc.2011.545"
        }
      }
    },
    abstract "BACKGROUND: Obtaining tissue for pancreatic carcinoma diagnosis
 and biomarker assessment to aid drug development is challenging. Circulating
 tumour cells (CTCs) may represent a potential biomarker to address these
 unmet needs. We compared prospectively the utility of two platforms for CTC
 enumeration and characterisation in pancreatic cancer patients in a pilot
 exploratory study. PATIENTS AND METHODS: Blood samples were obtained
 prospectively from 54 consenting patients and analysed by CellSearch and
 isolation by size of epithelial tumour cells (ISET). CellSearch exploits
 immunomagnetic capture of CTCs-expressing epithelial markers, whereas ISET is
 a marker independent, blood filtration device. Circulating tumour cell
 expression of epithelial and mesenchymal markers was assessed to explore any
 discrepancy in CTC number between the two platforms. RESULTS: ISET detected
 CTCs in more patients than CellSearch (93% vs 40%) and in higher numbers
 (median CTCs/7.5 ml, 9 (range 0-240) vs 0 (range 0-144)). Heterogeneity
 observed for epithelial cell adhesion molecule, pan-cytokeratin (CK),
 E-Cadherin, Vimentin and CK 7 expression in CTCs may account for discrepancy
 in CTC number between platforms. CONCLUSION: ISET detects more CTCs than
 CellSearch and offers flexible CTC characterisation with potential to
 investigate CTC biology and develop biomarkers for pancreatic cancer patient
 management.",
    mesh {
      {
        term "Adenocarcinoma",
        qual {
          {
            mp TRUE,
            subh "diagnosis"
          },
          {
            subh "mortality"
          },
          {
            subh "pathology"
          }
        }
      },
      {
        term "Adult"
      },
      {
        term "Aged"
      },
      {
        term "Aged, 80 and over"
      },
      {
        term "England"
      },
      {
        term "Female"
      },
      {
        term "Humans"
      },
      {
        term "Male"
      },
      {
        term "Middle Aged"
      },
      {
        mp TRUE,
        term "Neoplastic Cells, Circulating"
      },
      {
        term "Pancreatic Neoplasms",
        qual {
          {
            mp TRUE,
            subh "diagnosis"
          },
          {
            subh "mortality"
          },
          {
            subh "pathology"
          }
        }
      },
      {
        term "Pilot Projects"
      },
      {
        term "Predictive Value of Tests"
      },
      {
        term "Prospective Studies"
      },
      {
        term "Survival Analysis"
      },
      {
        mp TRUE,
        term "Tumor Markers, Biological"
      }
    },
    substance {
      {
        type nameonly,
        name "Tumor Markers, Biological"
      }
    },
    idnum {
      "c147/A12328a/Cancer Research UK"
    },
    pmid 22187035,
    pub-type {
      "Evaluation Studies",
      "Journal Article",
      "Research Support, Non-U.S. Gov't"
    },
    status medline
  }
}

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