Pubmed-entry ::= {
  pmid 22272297,
  medent {
    em std {
      year 2012,
      month 1,
      day 24
    },
    cit {
      title {
        name "Erythrocyte inosine triphosphatase activity is decreased in
 HIV-seropositive individuals."
      },
      authors {
        names ml {
          "Bierau J",
          "Bakker JA",
          "Schippers JA",
          "Grashorn JA",
          "Lindhout M",
          "Lowe SH",
          "Paulussen AD",
          "Verbon A"
        },
        affil str "Department of Clinical Genetics, Maastricht University
 Medical Centre, Maastricht, The Netherlands. jorgen.bierau@mumc.nl"
      },
      from journal {
        title {
          iso-jta "PLoS ONE",
          ml-jta "PLoS One",
          issn "1932-6203",
          name "PloS one"
        },
        imp {
          date std {
            year 2012
          },
          volume "7",
          issue "1",
          pages "E30175",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus received,
              date std {
                year 2011,
                month 5,
                day 27
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2011,
                month 12,
                day 13
              }
            },
            {
              pubstatus epublish,
              date std {
                year 2012,
                month 1,
                day 17
              }
            },
            {
              pubstatus other,
              date std {
                year 2012,
                month 1,
                day 25,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2012,
                month 1,
                day 25,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2012,
                month 1,
                day 25,
                hour 6,
                minute 0
              }
            }
          }
        }
      },
      ids {
        doi "10.1371/journal.pone.0030175",
        pii "PONE-D-11-09634",
        pubmed 22272297,
        other {
          db "pmc",
          tag str "PMC3260219"
        }
      }
    },
    abstract "BACKGROUND: Inosine triphosphatase (ITPase) is encoded by the
 polymorphic gene ITPA and maintains low intracellular levels of the inosine
 nucleotides ITP and dITP. The most frequently reported polymorphisms are ITPA
 c.94C>A (rs 1127354) and ITPA c. 124+21 A>C (rs7270101). Some
 nucleoside-analogues used in the treatment of HIV-seropositive (HIV+)
 patients are potential substrates for ITPase. Therefore, the frequency of
 ITPA SNPs and ITPase activity were studied in a population of HIV+-patients.
 METHODS: The study population consisted of 222 patients, predominantly
 Caucasian males, >95% using HAART. Erythrocyte ITPase activity was determined
 by measuring the formation of IMP from ITP. ITPA genotype was determined by
 sequencing genomic DNA. Distribution of ITPase activity, genotype-phenotype
 correlation and allele frequencies were compared to 198 control subjects. The
 effect of nucleoside analogues on ITPase activity was studied using
 lymphoblastic T-cell cultures and human recombinant ITPase. Enzyme kinetic
 experiments were performed on erythrocyte ITPase from HIV+ patients and
 controls. RESULTS: No difference was observed in the allele frequencies
 between the HIV+-cohort (+/- HAART) and the control population. HIV+ carriers
 of the wild type and ITPA c.94C>A had significantly lower ITPase activities
 than control subjects with the same genotype (pC carriers. Nucleoside analogues did not affect ITPase
 activity in cell culture and human recombinant ITPase. CONCLUSION: ITPA
 population genetics were identical in HIV+ and control populations. However,
 the majority of HIV+-patients had decreased erythrocyte ITPase activity
 compared to controls, probably due to decreased amounts of ITPase protein. It
 seems unlikely that ITPase activity is decreased due to nucleoside analogues
 (HAART). Long-term effects of HIV-infection altering ITPase protein
 expression or stability may explain the phenomenon observed.",
    pmid 22272297,
    pub-type {
      "Journal Article"
    },
    status premedline
  }
}

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