Pubmed-entry ::= {
  pmid 21946914,
  medent {
    em std {
      year 2011,
      month 12,
      day 14
    },
    cit {
      title {
        name "Recombinant human milk fat globule-EGF factor 8 produces
 dose-dependent benefits in sepsis."
      },
      authors {
        names ml {
          "Shah KG",
          "Wu R",
          "Jacob A",
          "Molmenti EP",
          "Nicastro J",
          "Coppa GF",
          "Wang P"
        },
        affil str "Department of Surgery, Hofstra North Shore-LIJ School of
 Medicine, 350 Community Drive, Manhasset, NY 11030, USA."
      },
      from journal {
        title {
          iso-jta "Intensive Care Med",
          ml-jta "Intensive Care Med",
          issn "1432-1238",
          name "Intensive care medicine"
        },
        imp {
          date std {
            year 2012,
            month 1
          },
          volume "38",
          issue "1",
          pages "128-136",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus received,
              date std {
                year 2010,
                month 10,
                day 29
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2011,
                month 8,
                day 2
              }
            },
            {
              pubstatus aheadofprint,
              date std {
                year 2011,
                month 9,
                day 23
              }
            },
            {
              pubstatus other,
              date std {
                year 2011,
                month 9,
                day 28,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2011,
                month 9,
                day 29,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2012,
                month 4,
                day 21,
                hour 6,
                minute 0
              }
            }
          }
        }
      },
      ids {
        doi "10.1007/s00134-011-2353-7",
        pubmed 21946914
      }
    },
    abstract "PURPOSE: Animal milk fat globule-EGF factor 8 (MFG-E8) has been
 shown to be beneficial in attenuating the inflammatory response in sepsis. In
 this study, we examined the effect of recombinant human MFG-E8 (rhMFG-E8) in
 an animal model of sepsis in an effort to develop it as a potential therapy
 against sepsis in humans. METHODS: Rats were subjected to sepsis by cecal
 ligation and puncture (CLP), and at 5 h post-CLP, they were given different
 doses of rhMFG-E8 (20, 40, 80, 160 mug/kg BW) in normal saline. At 20 h
 post-CLP, samples were collected for further analysis. A 10-day survival
 study was also performed. RESULTS: At 20 h after CLP, organ injury
 indicators, serum IL-6 and TNF-alpha, and plasma HMGB-1 levels were
 significantly increased as compared to sham-operated animals. Treatment with
 20 mug/kg rhMFG-E8 significantly reduced these levels. With higher doses,
 further reductions in AST and ALT (59-62%), creatinine (65-68%), and lactate
 (46-57%), and serum IL-6 and TNF-alpha were obtained. The 160 mug/kg dose
 produced the greatest reduction in serum TNF-alpha. With treatment with 20
 mug/kg rhMFG-E8, HMGB-1 levels decreased by 80%, returning back to sham
 values. In a 10-day survival study, vehicle-treated animals produced a 36%
 survival rate, while rhMFG-E8 significantly improved the survival rate to
 68-72%. Treatment with increasing doses of rhMFG-E8 significantly reduced the
 number of apoptotic cells detected and markedly attenuated the tissue damages
 observed in the lungs. CONCLUSIONS: These data suggest that recombinant human
 MFG-E8 is beneficial in ameliorating sepsis in an animal model of sepsis.",
    mesh {
      {
        term "Animals"
      },
      {
        term "Antigens, Surface",
        qual {
          {
            subh "administration & dosage"
          },
          {
            mp TRUE,
            subh "pharmacology"
          },
          {
            subh "therapeutic use"
          }
        }
      },
      {
        term "Biological Markers",
        qual {
          {
            subh "blood"
          }
        }
      },
      {
        term "Dose-Response Relationship, Drug"
      },
      {
        term "Glycolipids",
        qual {
          {
            mp TRUE,
            subh "therapeutic use"
          }
        }
      },
      {
        term "Glycoproteins",
        qual {
          {
            mp TRUE,
            subh "therapeutic use"
          }
        }
      },
      {
        term "HMGB1 Protein",
        qual {
          {
            subh "drug effects"
          }
        }
      },
      {
        term "Humans"
      },
      {
        term "Interleukin-6",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Male"
      },
      {
        term "Milk Proteins",
        qual {
          {
            subh "administration & dosage"
          },
          {
            subh "pharmacology"
          },
          {
            mp TRUE,
            subh "therapeutic use"
          }
        }
      },
      {
        term "Rats"
      },
      {
        term "Rats, Sprague-Dawley"
      },
      {
        term "Recombinant Proteins",
        qual {
          {
            mp TRUE,
            subh "therapeutic use"
          }
        }
      },
      {
        term "Sepsis",
        qual {
          {
            mp TRUE,
            subh "drug therapy"
          },
          {
            subh "pathology"
          }
        }
      },
      {
        term "Survival Analysis"
      },
      {
        term "Tumor Necrosis Factor-alpha",
        qual {
          {
            subh "drug effects"
          }
        }
      }
    },
    substance {
      {
        type nameonly,
        name "Antigens, Surface"
      },
      {
        type nameonly,
        name "Biological Markers"
      },
      {
        type nameonly,
        name "Glycolipids"
      },
      {
        type nameonly,
        name "Glycoproteins"
      },
      {
        type nameonly,
        name "HMGB1 Protein"
      },
      {
        type nameonly,
        name "Interleukin-6"
      },
      {
        type nameonly,
        name "Mfge8 protein, mouse"
      },
      {
        type nameonly,
        name "Milk Proteins"
      },
      {
        type nameonly,
        name "Recombinant Proteins"
      },
      {
        type nameonly,
        name "Tumor Necrosis Factor-alpha"
      },
      {
        type nameonly,
        name "milk fat globule"
      }
    },
    idnum {
      "R01GM053008/GM/NIGMS NIH HHS",
      "R01GM057468/GM/NIGMS NIH HHS"
    },
    pmid 21946914,
    pub-type {
      "Journal Article",
      "Research Support, N.I.H., Extramural"
    },
    status medline
  }
}

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