Pubmed-entry ::= {
  pmid 22357933,
  medent {
    em std {
      year 2012,
      month 3,
      day 7
    },
    cit {
      title {
        name "miR-125 potentiates early neural specification of human
 embryonic stem cells."
      },
      authors {
        names ml {
          "Boissart C",
          "Nissan X",
          "Giraud-Triboult K",
          "Peschanski M",
          "Benchoua A"
        },
        affil str "INSERM U861, AFM, Evry Cedex, France."
      },
      from journal {
        title {
          iso-jta "Development",
          ml-jta "Development",
          issn "1477-9129",
          name "Development (Cambridge, England)"
        },
        imp {
          date std {
            year 2012,
            month 4
          },
          volume "139",
          issue "7",
          pages "1247-1257",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus aheadofprint,
              date std {
                year 2012,
                month 2,
                day 22
              }
            },
            {
              pubstatus other,
              date std {
                year 2012,
                month 2,
                day 24,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2012,
                month 2,
                day 24,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2012,
                month 5,
                day 5,
                hour 6,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pii "dev.073627",
        doi "10.1242/dev.073627",
        pubmed 22357933
      }
    },
    abstract "The role of microRNAs (miRNAs) as coordinators of stem cell fate
 has emerged over the last decade. We have used human embryonic stem cells to
 identify miRNAs involved in neural lineage commitment induced by the
 inhibition of TGFbeta-like molecule-mediated pathways. Among several
 candidate miRNAs expressed in the fetal brain, the two isoforms of miR-125
 alone were detected in a time window compatible with a role in neural
 commitment in vitro. Functional analysis indicated that miR-125 isoforms were
 actively involved in the promotion of pluripotent cell conversion into
 SOX1-positive neural precursors. miR-125 promotes neural conversion by
 avoiding the persistence of non-differentiated stem cells and repressing
 alternative fate choices. This was associated with the regulation by miR-125
 of SMAD4, a key regulator of pluripotent stem cell lineage commitment.
 Activation of miR-125 was directly responsive to the levels of TGFbeta-like
 molecules, placing miR-125 at the core of mechanisms that lead to the
 irreversible neural lineage commitment of pluripotent stem cells in response
 to external stimuli.",
    mesh {
      {
        term "Activins",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Animals"
      },
      {
        term "Bone Morphogenetic Protein 4",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Cell Differentiation"
      },
      {
        term "Cell Line"
      },
      {
        term "Cell Separation"
      },
      {
        term "Embryonic Stem Cells",
        qual {
          {
            mp TRUE,
            subh "cytology"
          }
        }
      },
      {
        term "Gene Expression Regulation, Developmental"
      },
      {
        term "Humans"
      },
      {
        term "Mice"
      },
      {
        term "MicroRNAs",
        qual {
          {
            mp TRUE,
            subh "metabolism"
          }
        }
      },
      {
        term "Neurons",
        qual {
          {
            subh "cytology"
          },
          {
            mp TRUE,
            subh "metabolism"
          }
        }
      },
      {
        term "Pluripotent Stem Cells",
        qual {
          {
            subh "cytology"
          }
        }
      },
      {
        term "Protein Isoforms"
      },
      {
        term "Smad4 Protein",
        qual {
          {
            subh "metabolism"
          }
        }
      }
    },
    substance {
      {
        type nameonly,
        name "BMP4 protein, human"
      },
      {
        type nameonly,
        name "Bone Morphogenetic Protein 4"
      },
      {
        type nameonly,
        name "MIRN125 microRNA, human"
      },
      {
        type nameonly,
        name "MicroRNAs"
      },
      {
        type nameonly,
        name "Protein Isoforms"
      },
      {
        type nameonly,
        name "SMAD4 protein, human"
      },
      {
        type nameonly,
        name "Smad4 Protein"
      },
      {
        type cas,
        cit "104625-48-1",
        name "Activins"
      }
    },
    pmid 22357933,
    pub-type {
      "Journal Article",
      "Research Support, Non-U.S. Gov't"
    },
    status medline
  }
}

Other elisa kits Products