Pubmed-entry ::= {
  pmid 18405367,
  medent {
    em std {
      year 2008,
      month 4,
      day 29
    },
    cit {
      title {
        name "Gene expression pattern of functional neuronal cells derived
 from human bone marrow mesenchymal stromal cells."
      },
      authors {
        names ml {
          "Tondreau T",
          "Dejeneffe M",
          "Meuleman N",
          "Stamatopoulos B",
          "Delforge A",
          "Martiat P",
          "Bron D",
          "Lagneaux L"
        },
        affil str "Institut Jules Bordet, Universite Libre de Bruxelles,
 Laboratory of Experimental Hematology, 121, Bd de Waterloo, 1000 Brussels,
 Belgium. Tatiana.tondreau@bordet.be"
      },
      from journal {
        title {
          iso-jta "BMC Genomics",
          ml-jta "BMC Genomics",
          issn "1471-2164",
          name "BMC genomics"
        },
        imp {
          date std {
            year 2008
          },
          volume "9",
          pages "166",
          language "eng",
          pubstatus epublish,
          history {
            {
              pubstatus received,
              date std {
                year 2007,
                month 9,
                day 11
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2008,
                month 4,
                day 11
              }
            },
            {
              pubstatus aheadofprint,
              date std {
                year 2008,
                month 4,
                day 11
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2008,
                month 4,
                day 15,
                hour 9,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2008,
                month 6,
                day 17,
                hour 9,
                minute 0
              }
            },
            {
              pubstatus other,
              date std {
                year 2008,
                month 4,
                day 15,
                hour 9,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pii "1471-2164-9-166",
        doi "10.1186/1471-2164-9-166",
        pubmed 18405367,
        other {
          db "pmc",
          tag str "PMC2358905"
        }
      }
    },
    abstract "BACKGROUND: Neuronal tissue has limited potential to self-renew
 or repair after neurological diseases. Cellular therapies using stem cells
 are promising approaches for the treatment of neurological diseases. However,
 the clinical use of embryonic stem cells or foetal tissues is limited by
 ethical considerations and other scientific problems. Thus, bone marrow
 mesenchymal stomal cells (BM-MSC) could represent an alternative source of
 stem cells for cell replacement therapies. Indeed, many studies have
 demonstrated that MSC can give rise to neuronal cells as well as many
 tissue-specific cell phenotypes. METHODS: BM-MSC were differentiated in
 neuron-like cells under specific induction (NPBM + cAMP + IBMX + NGF +
 Insulin). By day ten, differentiated cells presented an expression profile of
 real neurons. Functionality of these differentiated cells was evaluated by
 calcium influx through glutamate receptor AMPA3. RESULTS: Using microarray
 analysis, we compared gene expression profile of these different samples,
 before and after neurogenic differentiation. Among the 1943 genes
 differentially expressed, genes down-regulated are involved in osteogenesis,
 chondrogenesis, adipogenesis, myogenesis and extracellular matrix component
 (tuftelin, AGC1, FADS3, tropomyosin, fibronectin, ECM2, HAPLN1, vimentin).
 Interestingly, genes implicated in neurogenesis are increased. Most of them
 are involved in the synaptic transmission and long term potentialisation as
 cortactin, CASK, SYNCRIP, SYNTL4 and STX1. Other genes are involved in
 neurite outgrowth, early neuronal cell development, neuropeptide
 signaling/synthesis and neuronal receptor (FK506, ARHGAP6, CDKRAP2, PMCH,
 GFPT2, GRIA3, MCT6, BDNF, PENK, amphiregulin, neurofilament 3, Epha4,
 synaptotagmin). Using real time RT-PCR, we confirmed the expression of
 selected neuronal genes: NEGR1, GRIA3 (AMPA3), NEF3, PENK and Epha4.
 Functionality of these neuron-like cells was demonstrated by Ca2+ influx
 through glutamate receptor channel (AMPA3) in the presence of two agonist
 glutamate, AMPA or CNQX antagonist. CONCLUSION: Our results demonstrate that
 BM-MSC have the potential to differentiate in neuronal cells with specific
 gene expression and functional properties. BM-MSC are thus promising
 candidates for cell-based therapy of neurodegenerative diseases.",
    mesh {
      {
        term "Bone Marrow Cells",
        qual {
          {
            mp TRUE,
            subh "metabolism"
          }
        }
      },
      {
        term "Cell Differentiation",
        qual {
          {
            subh "physiology"
          }
        }
      },
      {
        mp TRUE,
        term "Gene Expression Profiling"
      },
      {
        term "Humans"
      },
      {
        term "Mesenchymal Stem Cells",
        qual {
          {
            mp TRUE,
            subh "metabolism"
          }
        }
      },
      {
        term "Neurodegenerative Diseases",
        qual {
          {
            mp TRUE,
            subh "therapy"
          }
        }
      },
      {
        term "Neurons",
        qual {
          {
            subh "cytology"
          },
          {
            mp TRUE,
            subh "metabolism"
          }
        }
      },
      {
        term "Oligonucleotide Array Sequence Analysis"
      },
      {
        term "Stem Cell Transplantation",
        qual {
          {
            mp TRUE,
            subh "methods"
          }
        }
      },
      {
        term "Stromal Cells",
        qual {
          {
            mp TRUE,
            subh "metabolism"
          }
        }
      }
    },
    pmid 18405367,
    pub-type {
      "Comparative Study",
      "Journal Article",
      "Research Support, Non-U.S. Gov't"
    },
    status medline
  }
}

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