Pubmed-entry ::= {
  pmid 16142353,
  medent {
    em std {
      year 2005,
      month 9,
      day 5
    },
    cit {
      title {
        name "Gene expression profiling of a clonal isolate of
 oxaliplatin-resistant ovarian carcinoma cell line A2780/C10."
      },
      authors {
        names ml {
          "Varma RR",
          "Hector SM",
          "Clark K",
          "Greco WR",
          "Hawthorn L",
          "Pendyala L"
        },
        affil str "Roswell Park Cancer Institute, Buffalo, NY 14263, USA."
      },
      from journal {
        title {
          iso-jta "Oncol. Rep.",
          ml-jta "Oncol Rep",
          issn "1021-335X",
          name "Oncology reports"
        },
        imp {
          date std {
            year 2005,
            month 10
          },
          volume "14",
          issue "4",
          pages "925-932",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus pubmed,
              date std {
                year 2005,
                month 9,
                day 6,
                hour 9,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2006,
                month 2,
                day 4,
                hour 9,
                minute 0
              }
            },
            {
              pubstatus other,
              date std {
                year 2005,
                month 9,
                day 6,
                hour 9,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pubmed 16142353
      }
    },
    abstract "The efficacy of platinum drugs in the treatment of cancer is
 often restricted by the acquisition of tumor cell resistance subsequent to
 treatment. To better understand mechanisms involved in this phenomenon, a
 clonal subline (A2780/C10B) isolated from an oxaliplatin-resistant human
 ovarian carcinoma cell line (A2780/C10) was developed, as reported
 previously. This cell line is 18-fold resistant to oxaliplatin and shows a
 3-fold cross resistance to cisplatin. Here, we report on the gene expression
 analysis using Affymetrix HG-U95Av2 oligonucleotide arrays of cells in log
 phase growth from both the parental cell line and drug-resistant variant.
 Probe level analysis was perfomed using the model based expression index
 (dChip) and robust multichip average (RMA) methods. Genes that were
 differentially expressed between the two groups were identified using the
 significance analysis of microarrays (SAM) method with a minimum false
 discovery rate <1%. We identified 43 genes that were overexpressed, and 39
 underexpressed in the drug-resistant cell line. Collagen VI (COL6A3) was
 overexpressed 62-fold and the most highly up-regulated gene. This finding is
 consistent with other published data based on serial analysis of gene
 expression (SAGE) profiling of cisplatin-resistant and sensitive ovarian
 carcinoma cells. Among the significant functional groups of overexpressed
 genes in our study were extracellular matrix genes (9 of 43) and those
 involved in signal transduction (7 of 43). Extracellular matrix genes
 included two matrix metalloproteinases (MMP3 and MMP12). Integrin alpha 1
 (ITGA1) and WNT5A were also overexpressed. Genes that encode for
 extracellular matrix proteins were also among those found down-regulated in
 the resistant cell line. Several genes involved in the regulation of cell
 cycle and growth were found to be underexpressed, including the suppressor of
 cytokine signaling 2 (SOCS2), necdin (NDN), and glypicans (GPC3 and GPC4).
 The mRNA levels of six differentially expressed genes (COL6A3, MMP12, MMP3,
 WNT5A, NID, and HMGB2) were validated using real-time quantitative RT-PCR.
 The identification of these genes should aid in a better understanding of the
 pathways resulting in platinum drug resistance.",
    mesh {
      {
        term "Cell Line, Tumor"
      },
      {
        term "Cisplatin",
        qual {
          {
            subh "pharmacology"
          }
        }
      },
      {
        term "Collagen Type VI",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "DNA",
        qual {
          {
            subh "chemistry"
          }
        }
      },
      {
        term "DNA Repair"
      },
      {
        mp TRUE,
        term "Drug Resistance, Neoplasm"
      },
      {
        term "Extracellular Matrix",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Female"
      },
      {
        mp TRUE,
        term "Gene Expression Regulation, Neoplastic"
      },
      {
        term "HMGB2 Protein",
        qual {
          {
            subh "biosynthesis"
          }
        }
      },
      {
        term "Humans"
      },
      {
        term "Integrin alpha1",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Matrix Metalloproteinase 12"
      },
      {
        term "Matrix Metalloproteinase 3",
        qual {
          {
            subh "biosynthesis"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Membrane Glycoproteins",
        qual {
          {
            subh "biosynthesis"
          }
        }
      },
      {
        term "Metalloendopeptidases",
        qual {
          {
            subh "biosynthesis"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        mp TRUE,
        term "Oligonucleotide Array Sequence Analysis"
      },
      {
        term "Oligonucleotide Probes",
        qual {
          {
            subh "chemistry"
          }
        }
      },
      {
        term "Oligonucleotides",
        qual {
          {
            subh "chemistry"
          }
        }
      },
      {
        term "Organoplatinum Compounds",
        qual {
          {
            mp TRUE,
            subh "pharmacology"
          }
        }
      },
      {
        term "Ovarian Neoplasms",
        qual {
          {
            mp TRUE,
            subh "genetics"
          }
        }
      },
      {
        term "Proto-Oncogene Proteins",
        qual {
          {
            subh "biosynthesis"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "RNA, Messenger",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Reverse Transcriptase Polymerase Chain Reaction"
      },
      {
        term "Signal Transduction"
      },
      {
        term "Wnt Proteins",
        qual {
          {
            subh "biosynthesis"
          },
          {
            subh "metabolism"
          }
        }
      }
    },
    substance {
      {
        type nameonly,
        name "Collagen Type VI"
      },
      {
        type nameonly,
        name "HMGB2 Protein"
      },
      {
        type nameonly,
        name "Integrin alpha1"
      },
      {
        type nameonly,
        name "Membrane Glycoproteins"
      },
      {
        type nameonly,
        name "Oligonucleotide Probes"
      },
      {
        type nameonly,
        name "Oligonucleotides"
      },
      {
        type nameonly,
        name "Organoplatinum Compounds"
      },
      {
        type nameonly,
        name "Proto-Oncogene Proteins"
      },
      {
        type nameonly,
        name "RNA, Messenger"
      },
      {
        type nameonly,
        name "WNT5A protein, human"
      },
      {
        type nameonly,
        name "Wnt Proteins"
      },
      {
        type nameonly,
        name "nidogen"
      },
      {
        type cas,
        cit "15663-27-1",
        name "Cisplatin"
      },
      {
        type cas,
        cit "63121-00-6",
        name "oxaliplatin"
      },
      {
        type cas,
        cit "9007-49-2",
        name "DNA"
      },
      {
        type ec,
        cit "3.4.24.-",
        name "Metalloendopeptidases"
      },
      {
        type ec,
        cit "3.4.24.17",
        name "Matrix Metalloproteinase 3"
      },
      {
        type ec,
        cit "3.4.24.65",
        name "MMP12 protein, human"
      },
      {
        type ec,
        cit "3.4.24.65",
        name "Matrix Metalloproteinase 12"
      }
    },
    idnum {
      "CA16056/CA/NCI NIH HHS",
      "RR10742/RR/NCRR NIH HHS"
    },
    pmid 16142353,
    pub-type {
      "Journal Article",
      "Research Support, N.I.H., Extramural",
      "Research Support, U.S. Gov't, P.H.S."
    },
    status medline
  }
}

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