Pubmed-entry ::= {
  pmid 22504941,
  medent {
    em std {
      year 2012,
      month 5,
      day 9
    },
    cit {
      title {
        name "Mitochondrial genome sequencing of chondrocytes in
 osteoarthritis by             Human Mitochondria RT2 Profiler PCR Array."
      },
      authors {
        names ml {
          "Li Z",
          "Shen J",
          "Chen Y",
          "Pan J",
          "Zeng H",
          "Fang H",
          "Ye Z",
          "Zeng C",
          "Zhang R",
          "Cai D"
        },
        affil str "Department of Orthopaedics, The Third Affiliated Hospital,
 Sun                     Yat-sen University, Guangzhou, Guangdong 510630, P.R.
 China."
      },
      from journal {
        title {
          iso-jta "Mol Med Report",
          ml-jta "Mol Med Report",
          issn "1791-3004",
          name "Molecular medicine reports"
        },
        imp {
          date std {
            year 2012,
            month 7
          },
          volume "6",
          issue "1",
          pages "39-44",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus received,
              date std {
                year 2011,
                month 12,
                day 22
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2012,
                month 3,
                day 26
              }
            },
            {
              pubstatus aheadofprint,
              date std {
                year 2012,
                month 4,
                day 10
              }
            },
            {
              pubstatus other,
              date std {
                year 2012,
                month 4,
                day 17,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2012,
                month 4,
                day 17,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2012,
                month 4,
                day 17,
                hour 6,
                minute 0
              }
            }
          }
        }
      },
      ids {
        doi "10.3892/mmr.2012.863",
        pubmed 22504941,
        other {
          db "ELocationID doi",
          tag str "10.3892/mmr.2012.863"
        }
      }
    },
    abstract "Mitochondria are not only the main energy generators of the
 cell, but also             mediate several critical biochemical processes
 such as apoptosis, proliferation             and redox homeostasis. As such,
 mitochondrial dysfunctions can lead to a wide             variety of human
 diseases, including cancer and osteoarthritis (OA). In OA,
 mitochondrial-associated             signaling has been implicated in the
 molecular events leading to cartilage degradation,             including
 oxidative stress, defective chondrocyte biosynthesis and growth responses,   
          increased cytokine-induced chondrocyte inflammation and matrix
 catabolism, cartilage             matrix calcification and increased
 chondrocyte apoptosis. Thus, the mitochondrial             genome represents
 an attractive target for molecular therapy and OA research has            
 focused on determining its role in chondrocyte metabolism and subsequent
 cartilage             degradation. In this study, we analyzed the
 mitochondrial gene expression changes             that characterize
 chondrocytes in OA using the Human Mitochondria RT(2) Profiler            
 PCR Array. Twenty-six differentially expressed genes were identified that
 discriminated             chondrocytes in OA from those in normal cartilage,
 including 17 upregulated and             9 downregulated genes. These genes
 represent diverse functional categories, including             mitochondrial
 membrane polarization and potential, mitochondrial transport, small          
   molecule transport, targeting proteins to the mitochondria, mitochondrial
 protein             import, outer and inner membrane translocation,
 mitochondrial fission and fusion,             mitochondrial localization and
 apoptosis. Western blot analysis confirmed that             the p53
 upregulated modulator of apoptosis (PUMA; encoded by the BB3 gene) was       
      significantly upregulated in OA cartilage. In conclusion, our study
 generates             a differential mitochondrial gene expression profile
 for chondrocytes in OA and             demonstrates that mitochondrial genome
 dysregulation occurs in cartilage cells             during OA. Finally, our
 results indicate that PUMA may be a new diagnostic and            
 therapeutic target for OA.",
    pmid 22504941,
    pub-type {
      "Journal Article"
    },
    status premedline
  }
}

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