Pubmed-entry ::= {
  pmid 21813204,
  medent {
    em std {
      year 2011,
      month 10,
      day 31
    },
    cit {
      title {
        name "Serine protease inhibitor, 4-(2-aminoethyl)-benzene sulfonyl
 fluoride, impairs IL-12-induced activation of pSTAT4beta, NFkappaB, and
 select pro-inflammatory mediators from estrogen-treated mice."
      },
      authors {
        names ml {
          "Karpuzoglu E",
          "Gogal RM Jr",
          "Ansar Ahmed S"
        },
        affil str "College of Veterinary Medicine, Department of Anatomy and
 Radiology, University of Georgia, Athens, GA 30621, USA."
      },
      from journal {
        title {
          iso-jta "Immunobiology",
          ml-jta "Immunobiology",
          issn "1878-3279",
          name "Immunobiology"
        },
        imp {
          date std {
            year 2011,
            month 12
          },
          volume "216",
          issue "12",
          pages "1264-1273",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus received,
              date std {
                year 2010,
                month 10,
                day 5
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2011,
                month 7,
                day 3
              }
            },
            {
              pubstatus aheadofprint,
              date std {
                year 2011,
                month 7,
                day 7
              }
            },
            {
              pubstatus other,
              date std {
                year 2011,
                month 8,
                day 5,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2011,
                month 8,
                day 5,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2012,
                month 3,
                day 6,
                hour 6,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pii "S0171-2985(11)00134-3",
        doi "10.1016/j.imbio.2011.07.003",
        pubmed 21813204
      }
    },
    abstract "Estrogen, a natural immunomodulator, is believed to be involved
 in the regulation of not only normal immune responses, but also pathological
 conditions such as inflammatory and autoimmune diseases. We have previously
 reported that estrogen exposure induces several pro-inflammatory molecules
 including nitric oxide, cytokines and chemokines (IFNgamma, IL-12, MCP-1,
 etc.) and modifies transcription factors (preferential expression of
 STAT4beta, increased NFkappaB p50/p50 DNA binding, and enhanced T-bet and
 Bcl-3) from activated splenocytes. Given that estrogen promotes diverse range
 of pro-inflammatory molecules, and modifies transcription factors, it is
 plausible that estrogen upregulates a common set of molecular event(s) that
 favors inflammation. Serine proteases are thought to play an important role
 in inflammation. Therefore in this study, we investigated the consequence of
 exposure of splenocytes stimulated with a key Th1/IFNgamma-inducing cytokine
 IL-12 or ConA from estrogen-treated mice to a serine protease inhibitor,
 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), on inflammatory cytokines
 (IFNgamma, IL-12) and related transcription factors (STAT4alpha/beta, T-bet,
 NFkappaB). Exposure of splenocytes to AEBSF for 3h noticeably inhibited the
 induction of IFNgamma, IL-12, and IL-12-induced STAT4beta, mRNA expression of
 T-bet and IL-12Rbeta2. The AEBSF-mediated inhibition of cytokines was
 accompanied by the expression of a normal-sized NFkappaB, downregulation of
 p50/p50 DNA binding but did not alter Bcl3. These findings provide a new
 understanding of inflammation and inhibition of serine proteases has
 important implications for designing novel therapeutic strategies for a broad
 range of inflammatory diseases.",
    mesh {
      {
        term "Animals"
      },
      {
        term "Cells, Cultured"
      },
      {
        term "Cytokines",
        qual {
          {
            subh "genetics"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Estrogens",
        qual {
          {
            mp TRUE,
            subh "immunology"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Gene Expression Regulation",
        qual {
          {
            subh "drug effects"
          },
          {
            subh "immunology"
          }
        }
      },
      {
        term "Inflammation"
      },
      {
        term "Inflammation Mediators",
        qual {
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Mice"
      },
      {
        term "Mice, Inbred C57BL"
      },
      {
        term "NF-kappa B",
        qual {
          {
            subh "genetics"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Receptors, Interleukin-12",
        qual {
          {
            subh "genetics"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Serine Proteases",
        qual {
          {
            subh "immunology"
          },
          {
            mp TRUE,
            subh "metabolism"
          }
        }
      },
      {
        term "Serine Proteinase Inhibitors",
        qual {
          {
            mp TRUE,
            subh "pharmacology"
          }
        }
      },
      {
        term "Spleen",
        qual {
          {
            mp TRUE,
            subh "drug effects"
          },
          {
            subh "immunology"
          },
          {
            subh "metabolism"
          },
          {
            subh "pathology"
          }
        }
      },
      {
        term "Sulfones",
        qual {
          {
            mp TRUE,
            subh "pharmacology"
          }
        }
      },
      {
        term "T-Box Domain Proteins",
        qual {
          {
            subh "genetics"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Th1-Th2 Balance",
        qual {
          {
            subh "drug effects"
          }
        }
      }
    },
    substance {
      {
        type nameonly,
        name "Cytokines"
      },
      {
        type nameonly,
        name "Estrogens"
      },
      {
        type nameonly,
        name "Il12rb2 protein, mouse"
      },
      {
        type nameonly,
        name "Inflammation Mediators"
      },
      {
        type nameonly,
        name "NF-kappa B"
      },
      {
        type nameonly,
        name "Receptors, Interleukin-12"
      },
      {
        type nameonly,
        name "Serine Proteinase Inhibitors"
      },
      {
        type nameonly,
        name "Sulfones"
      },
      {
        type nameonly,
        name "T-Box Domain Proteins"
      },
      {
        type nameonly,
        name "T-box transcription factor TBX21"
      },
      {
        type cas,
        cit "34284-75-8",
        name "4-(2-aminoethyl)benzenesulfonylfluoride"
      },
      {
        type ec,
        cit "3.4.-",
        name "Serine Proteases"
      }
    },
    idnum {
      "5R01 AI51880-05/AI/NIAID NIH HHS"
    },
    pmid 21813204,
    pub-type {
      "Journal Article",
      "Research Support, N.I.H., Extramural",
      "Research Support, U.S. Gov't, Non-P.H.S."
    },
    status medline
  }
}

Other elisa kits Products