Pubmed-entry ::= {
  pmid 2440885,
  medent {
    em std {
      year 1987,
      month 9,
      day 17
    },
    cit {
      title {
        name "Radioimmunoassay for testicular cytochrome c (ct). Evidence for
 the presence of apocytochrome ct pool in rat testis extract."
      },
      authors {
        names ml {
          "Kim IC"
        }
      },
      from journal {
        title {
          iso-jta "J. Biol. Chem.",
          ml-jta "J Biol Chem",
          issn "0021-9258",
          name "The Journal of biological chemistry"
        },
        imp {
          date std {
            year 1987,
            month 8,
            day 15
          },
          volume "262",
          issue "23",
          pages "11156-11162",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus pubmed,
              date std {
                year 1987,
                month 8,
                day 15
              }
            },
            {
              pubstatus medline,
              date std {
                year 1987,
                month 8,
                day 15,
                hour 0,
                minute 1
              }
            },
            {
              pubstatus other,
              date std {
                year 1987,
                month 8,
                day 15,
                hour 0,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pubmed 2440885
      }
    },
    abstract "Mammalian testis contains a tissue-specific testicular
 cytochrome c (cyt ct). By immunizing rabbits with rat cyt ct and
 phosphorylated albumin (pBSA), rabbit anti-cyt ct was produced. Then the
 antiserum was applied to phosphorylated bovine serum albumin and rat somatic
 cyt c (cs)-Sepharose affinity columns to remove cross-reacting antibodies.
 The resultant anti-cyt ct was highly specific for cyt ct. From immunoblot
 assays, no protein other than cyt ct in rat testis extract was bound by the
 anti-cyt ct. By using the anti-cyt ct, radioimmunoassay (RIA) was developed
 for the quantitation of cyt ct in rat testis extract. The observation that
 the RIA did not bind rat cyt cs (1-1000 pmol), and other rat tissue extracts
 (kidney, heart, lung) further indicated that the RIA was highly specific for
 rat cyt ct. Separately, the concentration of holocyt ct was determined using
 CM-cellulose chromatography and subsequent spectral analysis on the same
 testis extract. The total cyt ct concentration in the rat testis extract
 determined by the RIA was about 3-fold higher than those determined by the
 latter techniques. Since the affinity purified anti-horse cyt c cross-reacted
 with both horse holo- and apocyt c, anti-rat cyt ct will cross-react with rat
 apocyt ct. Thus the concentration of cyt ct quantitated by the polyclonal
 anti-cyt ct-based RIA probably included apocyt ct concentration as well.
 Therefore, the higher cyt ct concentration determined by the RIA was probably
 attributed to the presence of the apocyt ct in the testis extract. The
 presence of the high concentration of the apocyt ct pool in testis is
 probably necessary to maintain continuous spermatogenesis, during which
 holocyt ct is incorporated into sperm mitochondria.",
    mesh {
      {
        term "Animals"
      },
      {
        term "Antibody Specificity"
      },
      {
        term "Apoproteins",
        qual {
          {
            mp TRUE,
            subh "analysis"
          }
        }
      },
      {
        term "Binding, Competitive"
      },
      {
        term "Chromatography"
      },
      {
        term "Cytochrome c Group",
        qual {
          {
            mp TRUE,
            subh "analysis"
          },
          {
            subh "immunology"
          }
        }
      },
      {
        term "Cytochromes c"
      },
      {
        term "Electrophoresis, Polyacrylamide Gel"
      },
      {
        term "Epitopes",
        qual {
          {
            subh "immunology"
          }
        }
      },
      {
        term "Horses"
      },
      {
        term "Immune Sera",
        qual {
          {
            subh "immunology"
          }
        }
      },
      {
        term "Immunoassay"
      },
      {
        term "Kidney",
        qual {
          {
            subh "analysis"
          }
        }
      },
      {
        term "Liver",
        qual {
          {
            subh "analysis"
          }
        }
      },
      {
        term "Male"
      },
      {
        term "Myocardium",
        qual {
          {
            subh "analysis"
          }
        }
      },
      {
        mp TRUE,
        term "Radioimmunoassay"
      },
      {
        term "Rats"
      },
      {
        term "Testis",
        qual {
          {
            mp TRUE,
            subh "analysis"
          }
        }
      }
    },
    substance {
      {
        type nameonly,
        name "Apoproteins"
      },
      {
        type nameonly,
        name "Cytochrome c Group"
      },
      {
        type nameonly,
        name "Epitopes"
      },
      {
        type nameonly,
        name "Immune Sera"
      },
      {
        type cas,
        cit "9007-43-6",
        name "Cytochromes c"
      }
    },
    idnum {
      "HD-17754/HD/NICHD NIH HHS"
    },
    pmid 2440885,
    pub-type {
      "Journal Article",
      "Research Support, U.S. Gov't, P.H.S."
    },
    status medline
  }
}

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