Pubmed-entry ::= {
pmid 17360961,
medent {
em std {
year 2007,
month 6,
day 21
},
cit {
title {
name "Vasoactive peptides in the luteolytic process activated by
PGF2alpha in pseudopregnant rabbits at different luteal stages."
},
authors {
names ml {
"Boiti C",
"Maranesi M",
"Dall'aglio C",
"Pascucci L",
"Brecchia G",
"Gobbetti A",
"Zerani M"
},
affil str "Dipartimento di Scienze Biopatologiche ed Igiene delle
Produzioni Animali e Alimentari, Sezione di Fisiologia veterinaria,
Laboratorio di Biotecnologie Fisiologiche, Universita degli Studi di Perugia,
I-06100 Perugia, Italy. cristiano.boiti@unipg.it"
},
from journal {
title {
iso-jta "Biol. Reprod.",
ml-jta "Biol Reprod",
issn "0006-3363",
name "Biology of reproduction"
},
imp {
date std {
year 2007,
month 7
},
volume "77",
issue "1",
pages "156-164",
language "eng",
pubstatus ppublish,
history {
{
pubstatus aheadofprint,
date std {
year 2007,
month 3,
day 14
}
},
{
pubstatus pubmed,
date std {
year 2007,
month 3,
day 16,
hour 9,
minute 0
}
},
{
pubstatus medline,
date std {
year 2007,
month 9,
day 26,
hour 9,
minute 0
}
},
{
pubstatus other,
date std {
year 2007,
month 3,
day 16,
hour 9,
minute 0
}
}
}
}
},
ids {
pii "biolreprod.106.055889",
doi "10.1095/biolreprod.106.055889",
pubmed 17360961
}
},
abstract "To study the role of endothelial factors in luteal function, the
dynamic profiles of genes for endothelin 1 (EDN1), its receptor subtypes,
EDNRA and EDNRB, and angiotensin converting enzyme (ACE) were examined in
corpora lutea (CL) obtained from rabbits on Days 4 and 9 of pseudopregnancy
after prostaglandin (PG) F2alpha analogue (alfaprostol) treatment. The cell
type distribution of EDN1 in the ovaries and its mechanisms of actions in
vitro and in vivo were also studied. Positive immunostaining for EDN1 was
localized in the luteal and endothelial cells, in granulosa cells of the
follicles, and in the ovarian epithelium. The basal mRNA levels for EDNRA,
EDNRB, and ACE were lower (P = 0.01) in Day-4 CL than in Day-9 CL, whereas
those for EDN1 did not differ between these two time-points. On Day 4, the
luteal EDN1, EDNRA, EDNRB, and ACE mRNA levels were similarly increased
two-fold (P = 0.01) 1.5 h after alfaprostol injection, and did not show
further changes in the subsequent 24 h. On Day 9, alfaprostol challenge
transiently up-regulated (P = 0.01) the luteal ACE transcripts at 1.5 h,
and those of EDN1 at 1.5 h and 3 h, whereas the EDNRA and EDNRB transcript
levels remained unchanged during the course of luteal regression. EDN1
decreased (P = 0.01) progesterone release and increased (P = 0.01)
PGF2alpha secretion and NOS activity via the PLC/PKC pathway in Day-9 CL, but
not in Day-4 CL, cultured in vitro. EDN1-induced, but not alfaprostol-induced
luteolysis, was blocked by cotreatment in vivo with the ACE antagonist
captopril. These findings support the hypothesis that PGF2alpha regulates
luteolysis through intraluteal activation of the renin-angiotensin/EDN1
systems in CL that have acquired luteolytic competence.",
mesh {
{
term "Animals"
},
{
term "Biological Markers"
},
{
term "Corpus Luteum",
qual {
{
mp TRUE,
subh "drug effects"
},
{
mp TRUE,
subh "physiology"
}
}
},
{
term "Dinoprost",
qual {
{
subh "metabolism"
},
{
mp TRUE,
subh "pharmacology"
}
}
},
{
term "Endothelin-1",
qual {
{
subh "metabolism"
}
}
},
{
term "Female"
},
{
term "Luteolysis",
qual {
{
mp TRUE,
subh "drug effects"
}
}
},
{
term "Ovary",
qual {
{
subh "cytology"
},
{
subh "metabolism"
}
}
},
{
term "Peptidyl-Dipeptidase A",
qual {
{
subh "metabolism"
}
}
},
{
term "Prostaglandins F",
qual {
{
mp TRUE,
subh "pharmacology"
}
}
},
{
term "Pseudopregnancy",
qual {
{
mp TRUE,
subh "metabolism"
}
}
},
{
term "Rabbits"
},
{
term "Receptors, Endothelin",
qual {
{
subh "metabolism"
}
}
}
},
substance {
{
type nameonly,
name "Biological Markers"
},
{
type nameonly,
name "Endothelin-1"
},
{
type nameonly,
name "Prostaglandins F"
},
{
type nameonly,
name "Receptors, Endothelin"
},
{
type cas,
cit "551-11-1",
name "Dinoprost"
},
{
type cas,
cit "74176-31-1",
name "alfaprostol"
},
{
type ec,
cit "3.4.15.1",
name "Peptidyl-Dipeptidase A"
}
},
pmid 17360961,
pub-type {
"Journal Article",
"Research Support, Non-U.S. Gov't"
},
status medline
}
}
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