Pubmed-entry ::= {
  pmid 20801358,
  medent {
    em std {
      year 2010,
      month 8,
      day 30
    },
    cit {
      title {
        name "A secondary antibody format chemiluminescence immunoassay for
 the determination of estradiol in human serum."
      },
      authors {
        names ml {
          "Xin TB",
          "Chen H",
          "Lin Z",
          "Liang SX",
          "Lin JM"
        },
        affil str "The Key Laboratory of Bioorganic Phosphorus Chemistry &
 Chemical Biology, Department of Chemistry, Tsinghua University, Beijing
 100084, China."
      },
      from journal {
        title {
          iso-jta "Talanta",
          ml-jta "Talanta",
          issn "1873-3573",
          name "Talanta"
        },
        imp {
          date std {
            year 2010,
            month 9,
            day 15
          },
          volume "82",
          issue "4",
          pages "1472-1477",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus received,
              date std {
                year 2010,
                month 5,
                day 31
              }
            },
            {
              pubstatus revised,
              date std {
                year 2010,
                month 7,
                day 8
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2010,
                month 7,
                day 9
              }
            },
            {
              pubstatus aheadofprint,
              date std {
                year 2010,
                month 7,
                day 16
              }
            },
            {
              pubstatus other,
              date std {
                year 2010,
                month 8,
                day 31,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2010,
                month 8,
                day 31,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2010,
                month 12,
                day 16,
                hour 6,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pii "S0039-9140(10)00537-0",
        doi "10.1016/j.talanta.2010.07.023",
        pubmed 20801358
      }
    },
    abstract "A competitive immunoassay for estradiol (E2) based on secondary
 antibody format was established. The donkey anti-rabbit IgG was used as the
 secondary antibody to coat micro-plates, and the horseradish peroxidase
 (HRP)-luminol-H(2)O(2) chemiluminescent system with high sensitivity was
 chosen as the detection system. The addition of sodium trichloroacetate
 (CCl(3)COONa) in the enzyme buffer as a replaceable packing material can
 realize directly analysis of E2 in human serum without extraction, which
 improved reproducibility and resolution of the assay. Additionally, the
 method showed specific recognition of estrogen, without cross-reaction for
 the major steroids (estrone (E1), estriol (E3), dihydrotestosterone (DHT),
 androstenedione, testosterone (T)) commonly found in human serum. The
 chemiluminescence immunoassay with secondary antibody can be applied to
 detect E2 with good precision at concentrations as low as 1.48 pg mL(-1). The
 proposed method has been successfully applied to the determination of E2 in
 97 human sera and showed a good correlation compared with the commercially
 radioimmunoassay (RIA) kit with a correlative coefficient of 0.9881. This
 method has exhibited great potential in the fabrication of diagnostic kit and
 can be used in the clinical analysis of E2 in human serum.",
    mesh {
      {
        term "Estradiol",
        qual {
          {
            mp TRUE,
            subh "blood"
          }
        }
      },
      {
        term "Humans"
      },
      {
        term "Immunoassay",
        qual {
          {
            mp TRUE,
            subh "methods"
          }
        }
      },
      {
        term "Kinetics"
      },
      {
        term "Luminescence"
      }
    },
    substance {
      {
        type cas,
        cit "50-28-2",
        name "Estradiol"
      }
    },
    pmid 20801358,
    pub-type {
      "Journal Article",
      "Research Support, Non-U.S. Gov't"
    },
    status medline
  }
}

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