Pubmed-entry ::= {
  pmid 16870304,
  medent {
    em std {
      year 2006,
      month 10,
      day 23
    },
    cit {
      title {
        name "Detailed analysis of the region 9-30 of rat follicle stimulating
 hormone receptor: identification of peptide 20-30 as a potential hormone
 binding inhibitor."
      },
      authors {
        names ml {
          "Ghosalkar JD",
          "Mahale SD"
        },
        affil str "Division of Structural Biology, National Institute for
 Research in Reproductive Health (Indian Council of Medical Research),
 Jehangir Merwanji Street, Parel, Mumbai 400012, India."
      },
      from journal {
        title {
          iso-jta "Peptides",
          ml-jta "Peptides",
          issn "0196-9781",
          name "Peptides"
        },
        imp {
          date std {
            year 2006,
            month 11
          },
          volume "27",
          issue "11",
          pages "2894-2900",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus received,
              date std {
                year 2006,
                month 4,
                day 11
              }
            },
            {
              pubstatus revised,
              date std {
                year 2006,
                month 5,
                day 23
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2006,
                month 5,
                day 23
              }
            },
            {
              pubstatus aheadofprint,
              date std {
                year 2006,
                month 7,
                day 25
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2006,
                month 7,
                day 28,
                hour 9,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2007,
                month 5,
                day 11,
                hour 9,
                minute 0
              }
            },
            {
              pubstatus other,
              date std {
                year 2006,
                month 7,
                day 28,
                hour 9,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pii "S0196-9781(06)00261-0",
        doi "10.1016/j.peptides.2006.05.027",
        pubmed 16870304
      }
    },
    abstract "The extracellular domain (ECD) of the follicle stimulating
 hormone receptor (FSHR) has been shown to be a major determinant of hormone
 selectivity. The N-terminal 9-30 region, the sequence of which is unique to
 FSHR, has been extensively studied earlier and has been proposed to be an
 FSHR neutralizing epitope. In this study antipeptide antibodies specific to
 the peptide 9-30 were generated and used for identifying a specific
 immunodominant region within it. Overlapping peptides corresponding to the
 regions 9-19, 15-25 and 20-30 were synthesized. The ability of the
 antipeptide antibodies to 9-30 of FSHR to bind to different peptides was
 checked. The results indicated that the antibodies mainly recognized the
 peptide 20-30 and not the other two overlapping peptides. Further, the effect
 of the peptide 20-30 on the binding of radiolabeled FSH to its receptor was
 monitored. This peptide showed FSH-binding inhibitory activity with an IC(50)
 value of 0.598 x 10(-4)M and was more effective than the peptide 9-30 itself.
 Binding kinetics revealed that the observed effect of the peptide 20-30 is
 due to mixed type of inhibitory mechanism. This is the smallest peptide from
 the rat FSHR sequence having ability to inhibit FSH binding to its receptor
 by more than 90%.",
    mesh {
      {
        term "Amino Acid Sequence"
      },
      {
        term "Animals"
      },
      {
        term "Antibodies",
        qual {
          {
            subh "pharmacology"
          }
        }
      },
      {
        term "Antibody Specificity"
      },
      {
        term "Binding Sites",
        qual {
          {
            subh "physiology"
          }
        }
      },
      {
        term "Binding, Competitive",
        qual {
          {
            subh "drug effects"
          },
          {
            subh "immunology"
          }
        }
      },
      {
        term "Blotting, Western"
      },
      {
        term "Epitope Mapping"
      },
      {
        term "Humans"
      },
      {
        term "Immunodominant Epitopes"
      },
      {
        term "Molecular Sequence Data"
      },
      {
        term "Peptide Fragments",
        qual {
          {
            mp TRUE,
            subh "chemistry"
          },
          {
            subh "genetics"
          },
          {
            subh "immunology"
          },
          {
            subh "isolation & purification"
          },
          {
            subh "metabolism"
          }
        }
      },
      {
        term "Protein Binding"
      },
      {
        term "Rabbits"
      },
      {
        term "Rats"
      },
      {
        term "Receptors, FSH",
        qual {
          {
            mp TRUE,
            subh "chemistry"
          },
          {
            subh "genetics"
          },
          {
            subh "immunology"
          },
          {
            subh "metabolism"
          }
        }
      }
    },
    substance {
      {
        type nameonly,
        name "Antibodies"
      },
      {
        type nameonly,
        name "Immunodominant Epitopes"
      },
      {
        type nameonly,
        name "Peptide Fragments"
      },
      {
        type nameonly,
        name "Receptors, FSH"
      },
      {
        type nameonly,
        name "follicle stimulating hormone receptor (20-30), rat"
      },
      {
        type nameonly,
        name "follicle stimulating hormone receptor (9-30), rat"
      }
    },
    pmid 16870304,
    pub-type {
      "Journal Article",
      "Research Support, Non-U.S. Gov't"
    },
    status medline
  }
}

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