Pubmed-entry ::= {
  pmid 22118483,
  medent {
    em std {
      year 2011,
      month 11,
      day 28
    },
    cit {
      title {
        name "Osteogenic capability of autologous rabbit adipose-derived
 stromal cells in repairing calvarial defects."
      },
      authors {
        names ml {
          "Cheng SW",
          "Lin ZQ",
          "Wang W",
          "Zhang W",
          "Kou DQ",
          "Ying XZ",
          "Chen QY",
          "Shen Y",
          "Cheng XJ",
          "Peng L",
          "Lv CZ"
        },
        affil str "Department of Orthopaedics, Second Affiliated Hospital of
 Wenzhou Medical College, Wenzhou 325000."
      },
      from journal {
        title {
          iso-jta "Chin. J. Traumatol.",
          ml-jta "Chin J Traumatol",
          issn "1008-1275",
          name "Chinese journal of traumatology = Zhonghua chuang shang za zhi
 / Chinese Medical Association"
        },
        imp {
          date std {
            year 2011,
            month 10,
            day 1
          },
          volume "14",
          issue "5",
          pages "288-292",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus other,
              date std {
                year 2011,
                month 11,
                day 29,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2011,
                month 11,
                day 29,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2011,
                month 11,
                day 29,
                hour 6,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pubmed 22118483
      }
    },
    abstract "Objective:  To evaluate the in vitro and in vivo osteogenic
 capability of adipose-derived stromal cells (ASCs).  Methods:    ASCs were
 isolated from New Zealand white rabbits and determined by alkaline
 phosphatase (ALP) staining, von Kossa staining and alizarin red staining.
 Some specific markers of osteogenic differentiation, including ALP,
 osteocalcin (OCN), osteopontin (OPN) were examined by reverse
 transcription-polymerase chain reaction (RT-PCR). In vivo, demineralized bone
 matrix (DBM)-ASCs composites were implanted into the rabbit calvarial defects
 created at each side of the longitudinal midline. After 6 weeks, histologic
 properties of the transplants were analyzed.  Results:    ASCs were
 successfully induced into osteogenesis. ALP staining, von Kossa staining and
 alizarin red staining showed positive results. The expressions of ALP, OCN
 and OPN were detected in ASCs after cultivation in osteogenic medium.
 Extensive new bone was observed in the defects transplanted with DBM-ASCs
 composites. Conclusion:    ASCs have the potential to differentiate into
 osteogenic lineage and DBM-ASCs constructs are a promising method for
 regeneration in bone defects.",
    pmid 22118483,
    pub-type {
      "Journal Article"
    },
    status premedline
  }
}

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