Pubmed-entry ::= {
  pmid 10321706,
  medent {
    em std {
      year 1999,
      month 7,
      day 15
    },
    cit {
      title {
        name "Prostaglandin E1 and recombinant bone morphogenetic protein
 effect on strength of hydroxyapatite implants."
      },
      authors {
        names ml {
          "Ono I",
          "Tateshita T",
          "Kuboki Y"
        },
        affil str "Department of Dermatology, Fukushima Medical University,
 School of Medicine, Japan."
      },
      from journal {
        title {
          iso-jta "J. Biomed. Mater. Res.",
          ml-jta "J Biomed Mater Res",
          issn "0021-9304",
          name "Journal of biomedical materials research"
        },
        imp {
          date std {
            year 1999,
            month 6,
            day 15
          },
          volume "45",
          issue "4",
          pages "337-344",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus pubmed,
              date std {
                year 1999,
                month 5,
                day 13,
                hour 2,
                minute 5
              }
            },
            {
              pubstatus medline,
              date std {
                year 2000,
                month 6,
                day 20,
                hour 9,
                minute 0
              }
            },
            {
              pubstatus other,
              date std {
                year 1999,
                month 5,
                day 13,
                hour 2,
                minute 5
              }
            }
          }
        }
      },
      ids {
        pubmed 10321706,
        pii "10.1002/(SICI)1097-4636(19990615)45:43.0.CO;2-H"
      }
    },
    abstract "Although combinations of hydroxyapatite (HAP) and bone
 morphogenetic protein (BMP) are expected to provide potent alternatives to
 autogenous bone grafts, it is still anticipated that substances that act
 synergistically with BMP will be found because the inducing potential of
 purified BMP in bone is not strong enough. We already have shown that
 prostaglandin (PG) E1 has a strong and dose-dependent synergistic effect on
 the osteoinductive activity induced by recombinant human (rh) BMP and that it
 enhances osteoconduction even when used alone. In this study, porous HAP rods
 were treated as follows: (1) without PGE1 or rhBMP (control group); (2) with
 varying concentrations of PGE1; and (3) with varying concentrations of PGE1
 combined with 1 microg of rhBMP-2. The rods were subperiosteally implanted on
 the cranial bone of rabbits to evaluate the effect of these treatments on the
 mechanical strength of the implanted HAP rods. The HAP rods were removed 3,
 6, or 9 weeks after implantation and subjected to mechanical strength
 determinations. The control group (no addition of BMP to the rods) showed no
 significant increase in three-point bending strength or in compression
 strength compared to pre-implantation. On the other hand, PGE1 combined with
 rhBMP had a strong and dose-dependent effect on the mechanical strength of
 HAP, increasing it significantly, especially compression strength. PGE1 also
 increased mechanical strength even when used alone. Histological examination
 revealed that PGE1, whether or not it was combined with rhBMP, increased bone
 formation into the pores of HAP and consequently increased the mechanical
 strength of porous HAP.",
    mesh {
      {
        term "Alprostadil",
        qual {
          {
            mp TRUE,
            subh "pharmacology"
          }
        }
      },
      {
        term "Animals"
      },
      {
        mp TRUE,
        term "Biocompatible Materials"
      },
      {
        term "Bone Development",
        qual {
          {
            mp TRUE,
            subh "drug effects"
          }
        }
      },
      {
        term "Bone Morphogenetic Proteins",
        qual {
          {
            mp TRUE,
            subh "pharmacology"
          }
        }
      },
      {
        term "Bone and Bones",
        qual {
          {
            subh "anatomy & histology"
          },
          {
            subh "drug effects"
          }
        }
      },
      {
        term "Drug Synergism"
      },
      {
        term "Humans"
      },
      {
        mp TRUE,
        term "Hydroxyapatites"
      },
      {
        term "Rabbits"
      },
      {
        term "Recombinant Proteins",
        qual {
          {
            subh "pharmacology"
          }
        }
      },
      {
        term "Tensile Strength"
      },
      {
        term "Time Factors"
      }
    },
    substance {
      {
        type nameonly,
        name "Biocompatible Materials"
      },
      {
        type nameonly,
        name "Bone Morphogenetic Proteins"
      },
      {
        type nameonly,
        name "Hydroxyapatites"
      },
      {
        type nameonly,
        name "Recombinant Proteins"
      },
      {
        type cas,
        cit "745-65-3",
        name "Alprostadil"
      }
    },
    pmid 10321706,
    pub-type {
      "Journal Article"
    },
    status medline
  }
}

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