Pubmed-entry ::= {
  pmid 22553577,
  medent {
    em std {
      year 2012,
      month 5,
      day 3
    },
    cit {
      title {
        name "Inhibition of corneal neovascularization by vascular endothelia
 growth inhibitor gene."
      },
      authors {
        names ml {
          "Wang H",
          "Wang B"
        },
        affil str "Department of Ophthalmology, the Provincial Hospital
 Affiliated to Shandong University, Jinan 250021, Shandong Province, China."
      },
      from journal {
        title {
          iso-jta "Int J Ophthalmol",
          ml-jta "Int J Ophthalmol",
          issn "2222-3959",
          name "International journal of ophthalmology"
        },
        imp {
          date std {
            year 2010
          },
          volume "3",
          issue "4",
          pages "295-298",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus received,
              date std {
                year 2010,
                month 11,
                day 9
              }
            },
            {
              pubstatus accepted,
              date std {
                year 2010,
                month 11,
                day 28
              }
            },
            {
              pubstatus epublish,
              date std {
                year 2010,
                month 12,
                day 18
              }
            },
            {
              pubstatus other,
              date std {
                year 2012,
                month 5,
                day 4,
                hour 6,
                minute 0
              }
            },
            {
              pubstatus pubmed,
              date std {
                year 2010,
                month 1,
                day 1,
                hour 0,
                minute 0
              }
            },
            {
              pubstatus medline,
              date std {
                year 2010,
                month 1,
                day 1,
                hour 0,
                minute 0
              }
            }
          }
        }
      },
      ids {
        doi "10.3980/j.issn.2222-3959.2010.04.04",
        pii "ijo-03-04-295",
        pubmed 22553577,
        other {
          db "pmc",
          tag str "PMC3340739"
        }
      }
    },
    abstract "AIM: To evaluate the effect of Effectene lipofectine mediated
 plasmids encoding human pcDNA(4)-vascular endothelia growth inhibitor
 (pcDNA(4)-VEGI) gene on corneal neovascularization (CNV). METHODS: Forty New
 Zealand albino rabbits were sutured by 5-0 silk on the superior cornea to
 induce CNV and divided into 4 random teams, ten per each team: team A:
 transfected by pcDNA(4)-VEGI gene mediated by Effectene() lipofectine
 transfection; team B: by plasmid pcDNA(4); team C: by Effectene(), and team
 D: by normal saline. Length and area of CNV were observed under slit lamp
 every day after transfection. Immunohistochemistry was performed to detect
 the expression of VEGI protein in corneas at day 3, 7, 14 and 21. RESULTS: 1)
 Average occurrence of CNV was 6.3 days in team A, 3.1 days in team B, 3.2
 days in team C, and 3.2 days in team D. Difference was significant between A
 and other teams (P<0.01); 2) Length and average area of CNV in each period in
 team A was significantly different from those in team B, C and D (P<0.01); 3)
 VEGI expressions were observed in epithelium, stroma, endothelium and the
 cliff of CNV in team A at 3 days after transfection by immunohistochemical
 staining. None VEGI positive cells were found in the control teams (team B, C
 and D) all the time. CONCLUSION: Effectene() lipofectine transfection
 technique can effectively transfect pcDNA(4)-VEGI gene into rabbit cornea and
 the length and CNV areas can be inhibited by VEGI gene.",
    pmid 22553577,
    pub-type {
      "Journal Article"
    },
    status premedline
  }
}

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