Pubmed-entry ::= {
  pmid 2554758,
  medent {
    em std {
      year 1989,
      month 12,
      day 12
    },
    cit {
      title {
        name "Quantitation of testicular and somatic cytochromes c in testis
 and somatic tissues from developing rats."
      },
      authors {
        names ml {
          "Kim IC"
        },
        affil str "Division of Biomedical Research, Lovelace Medical
 Foundation, Albuquerque, New Mexico 87108."
      },
      from journal {
        title {
          iso-jta "Anal. Biochem.",
          ml-jta "Anal Biochem",
          issn "0003-2697",
          name "Analytical biochemistry"
        },
        imp {
          date std {
            year 1989,
            month 8,
            day 15
          },
          volume "181",
          issue "1",
          pages "140-144",
          language "eng",
          pubstatus ppublish,
          history {
            {
              pubstatus pubmed,
              date std {
                year 1989,
                month 8,
                day 15
              }
            },
            {
              pubstatus medline,
              date std {
                year 1989,
                month 8,
                day 15,
                hour 0,
                minute 1
              }
            },
            {
              pubstatus other,
              date std {
                year 1989,
                month 8,
                day 15,
                hour 0,
                minute 0
              }
            }
          }
        }
      },
      ids {
        pubmed 2554758,
        pii "0003-2697(89)90407-7"
      }
    },
    abstract "By combining chromatographic and spectral procedures, simple and
 quantitative assays for somatic cytochrome c (cyt cs) and testicular
 cytochrome c (cyt ct) in crude animal tissue extracts were developed. Using
 this assay procedure, limited developmental studies of cyt ct and cs were
 performed with tissue extracts of 27-, 58-, and 85-day-old rats. Specific
 contents of cyt cs in somatic tissues (i.e., micrograms of cyt c/g of tissue)
 of these three age groups did not show significant variations. However, the
 amounts of both cyt ct and cs in testis were markedly increased as the rats
 approached maturity. Increasing cyt ct/cyt cs ratios as the rat developed to
 maturity suggest that expression of cyt ct is preferentially required for
 specific function of testis. Application of both molecular biological
 techniques and this assay (for holo-cyt ct) should be useful to study the
 overall regulation of the expression of cyt ct in testis.",
    mesh {
      {
        term "Aging",
        qual {
          {
            subh "physiology"
          }
        }
      },
      {
        term "Animals"
      },
      {
        term "Carboxymethylcellulose Sodium",
        qual {
          {
            subh "diagnostic use"
          }
        }
      },
      {
        term "Chromatography",
        qual {
          {
            subh "methods"
          }
        }
      },
      {
        term "Cytochrome c Group",
        qual {
          {
            mp TRUE,
            subh "analysis"
          }
        }
      },
      {
        term "Male"
      },
      {
        term "Rats"
      },
      {
        term "Rats, Inbred Strains"
      },
      {
        term "Sexual Maturation"
      },
      {
        term "Testis",
        qual {
          {
            mp TRUE,
            subh "analysis"
          }
        }
      }
    },
    substance {
      {
        type nameonly,
        name "Cytochrome c Group"
      },
      {
        type cas,
        cit "9004-32-4",
        name "Carboxymethylcellulose Sodium"
      }
    },
    idnum {
      "HD-17754/HD/NICHD NIH HHS"
    },
    pmid 2554758,
    pub-type {
      "Journal Article",
      "Research Support, U.S. Gov't, P.H.S."
    },
    status medline
  }
}

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